Composite

Part:BBa_K1890011:Design

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-10-07)


mVenus with strong consitutive promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimized for E. coli.

Source

Derived from Aequorea Victoria

References

[1] Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90 (2002).